Clean up Check that your data in Table 3 increases linearly before cleaning up your experiment. Spectrophotometric determination of iron in Spectrophotometric determination of iron in Spectrophotometric Determination of Iron in Dietary Tablets Objectives: Spectrophotometry, because of its simplicity, selectivity, sensitivity and cost-effectiveness, continues to be widely used in pharmaceutical analysis including in the assay of phenothiazines.
After this, you are ready to measure your sample use 0. Transfer 1 mL of the digested unknown solution into a mL volumetric flask and add sodium citrate until the solution pH is around 3. Fe II and 0. The absorbance of each solution in nm was identified after all of the standard solutions, sample and blank was diluted to mark.
In your final calculations, you will need to scale up your final iron concentration in the pill to account for any powder that you did not use. Mostof these classical detectors are coupled with flow injectionanalysis FIA or sequential injection analysis SIA andare used for quantification of elements in a variety of realsamples.
The relationship obtained between the rel-ative peak height and iron II concentration is given by theequation. Fe II was determined by SIA based onthe reaction between 1,phenanthroline and iron IIyielding an orangered colour complex with absorption maximum at nm.
Add approximately mL of 1. In mL volumetric flasks prepare a series of unknown solutions containing 1. Due to the spreading of data, the computed standard deviation of the results in iron is around Determine the mass of the pill.
Sodium acetate NaC2H3O2 is used to control the pH of the solution since this also affects the absorbance. First the molecules rotates about various axes, the energy of rotation being at definite levels, so the molecule may absorbed radiation and be raised to a higher rotational energy level, in a rotational transition.
Using a Spectronic at nm, read and record the Absorbance A of your solutions. Pour the iron standard solutions down the drain. For the future experiment, it is recommended to use a volumetric pipette when transferring any amount of liquid from a volumetric flask to a volumetric flask. We will use this method in our iron determination.
There are three basic processes by which a molecule can absorbed radiation by raising the molecule to a higher internal energy level, the increase in energy being equal to the energy of the absorbed radiation.
Sequential injection spectrophotometric determination of iron as Fe II in multi-vitamin preparations using 1,phenanthroline as complexing agentIntroductionExperimentalReagents and solutionsApparatusSample preparationProcedureResults and discussionOptimisation of parametersFlow rateSample volume and reagent volume optimisationSample volumeReagent volumeCoil length and diameterReaction coilHolding coilConcentration of reagentMethod evaluationLinearity, detection limit, accuracy and precisionInterferencesStatistical comparisonConclusionReferences Recommended.
The percent error is the absolute difference between the experimental and theoretical value divided by the theoretical value, then multiplied by to get a percent. Another source of error is because volumetric pipettes were not used in transferring liquids from one Experiment 1.
The influence of reagent vol-ume on response and precision was studied between 93and l at constant sample volume and the resultsare given in Fig. Results and Discussions Analyzing the amount of iron in the vitamin tablet through the use of UV-VIS Spectrophotometer is the main objective of the experiment.
Theoptimum condition was evaluated and the calibration curve is linear over a range of 0. The dilution could also contribute to the error in the analysis. Influence of reagent concentration on sensitivity and precision.
Working standard solu-tions ranging between 0.
Wait 10 minutes for the reaction to complete. The colorimetric reagent of 0. In mL volumetric flasks prepare a series of standard solutions containing 1.
In your final calculations, you will need to scale up your final iron concentration in the pill to account for any powder that you did not use.
Determination of maximum wavelength Figure 4 shows the UV-VIS spectrum used in the determination of the maximum wavelength in the experiment through the use of the instrument Spectronic Genesys 5. The comparison of the intensity of the color of solutions of known concentration with the intensity of an unknown could be a tool in identification of the concentration of the unknown solution.
You can then compare this value to the amount listed on the bottle in terms of percent error. Construction of calibration curve. By using flow reversal the stack of well-definedzones were propelled by the peristaltic pump from the hold-ing coil to the reaction coil.
Based on the result of experiment, each tablet contains approximately 83 mg of iron and mg of ferrous sulfate. In an attempt to overcome this limitation, the author has simplified the above procedure by using dichromate as the oxidant. The apparent molar absorptivity values are in the range 3.
It was observed that, the sensitivity steadily increases withan increase in the concentration of the reagent and the re-sults are given in Fig.Spectrophotometric Determination of Iron in Vitamin Tablets In this procedure, iron from a vitamin supplement tablet is dissolved in acid, reduced to Fe2+ with hydroquinone, and complexed with o-phenanthroline to form an intensely colored complex.
The apparent molar absorptivity values are in the range 10 10 3 L mol-1 cm-1 and the limits of determination range from to g mL-1 and the relative standard deviation is less than 2%. The proposed method has been applied for the determination of these drugs in pure form and in pharmaceutical formulations with recoveries in.
1 I. INTRODUCTION The organic compound 1,phenanthroline has been used since as a reagent for the spectrophotometric determination of small amounts of iron.
Spectrophotometric Determination of Iron with 1,Phenanthroline in the Presence of Copper Use of Triethylenetetramine as Masking Agent By Zhenpu Wang" and K. L. Cheng With 3 Figures (Received May 20, ) 1,Phenanthroline is well known for determining traces of iron.
Indirect spectrophotometric determination of phenothiazine and pH in which iron(III) was used as the oxidant and the iron(II) formed was measured as its 1,phenanthroline complex at nm, has been reported by Buhl and Chwistek DOI: /JAPS ISSN Validation of sensitive spectrophotometric method for determination of Salmeterol xinafoate and Fenoterol hydrobromide via o-Phenanthroline and iron complexation Mohamed A.
Hammad 1, Mahmoud A. Omar, Mohamed Awad2* 1Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt.Download